The technology disclosure introduces a method for detecting expression of c-kit receptor, which is crucial in various diseases including cancer, allergies, and autoimmune disorders.

This antibody-based detection method utilizes self-labeled fusion proteins, consisting of stem cell factor (SCF) fused to SNAP-tag, allowing for covalent binding to detectable agent.

The Process Involves Following Steps:

Genetic Engineering:

• Fusion constructs of SCF fused to a SNAP tag are cloned into a mammalian vector.

Transfection:

• The fusion protein probe is transfected into host cells for genetic expression.

Purification:

• The expressed fusion protein probe is purified from the transfected culture.

Labeling:

• The probe is labeled with a detectable agent, typically benzylguanine or a derivative thereof with a fluorophore.