Industrial Consultancy & Sponsored Research (IC&SR) , IIT Madras
Method For Preparing Calcium Lactate Nanostructures and its Applications thereof
Technology Category/Market
Technology: Method for Preparing Calcium Lactate Nanostructures
Category: Biotechnology & Genetic Engineering
Industry: Biomedical
Application: Drug Treatment of cancer
Market: The global market size was valued at USD 201.75 billion in 2023 and is projected to grow from USD 220.80 billion in 2024 to USD 518.25 billion by 2032, exhibiting a CAGR of 11.3% during the forecast period (2024-2032).
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Problem Statement
- RNA interference (RNAi) involves introducing a short dsRNA into cells to silence gene expression.
- The RNA-induced silencing complex (RISC) recognizes and binds to target mRNA, inhibiting translation or protein expression.
- Essential in gene-based therapeutics for diseases like cancer, microbial infections, neurological disorders, immune-mediated diseases, and genetic disorders.
- Challenges include safe, efficient, and economical delivery of siRNA molecules due to their anionic nature, low stability, and short half-life.
- Need for improved calcium lactate nanostructure-based transfection reagent for effective gene silencing and therapeutic applications.
Technology
A method for preparing calcium lactate nanostructures, which are used as sirna delivery vehicles in various therapeutic applications, including rnai-based therapeutics, targeted drug delivery, and combination therapy for various disease treatments.
Key Features/Value Proposition
Preparing Calcium Lactate Nanostructures for Therapeutic Delivery:
- Addition of calcium carbonate nanoparticles to 40% lactic acid for sonication and stirring.
- Addition of ethanol to the syrupy liquid to form a white slurry with calcium lactate nanostructures.
- Centrifugation at 12000 rpm for 20 minutes at 20°C to retain sediment and remove supernatant.
- Washing of sediment with 90% ethanol for obtaining calcium lactate nanostructures.
- Lyophilization and storage at -80°C for obtaining nanostructures.
- Nanostructures are crystalline nanostrip, nanobelt, nanowire, nanorods with positive zeta potential.
Calcium Lactate Nanostructure Complexation Method
- Complexes calcium lactate nanostructure with at least one siRNA.
- Complexing occurs at pH 5-8, temperature 15-37°C, and incubation period ranging from seconds to 10 minutes.
- CaLNS-siRNA complex inhibits target gene expression via RNA interference.
Calcium Carbonate Nanoparticle Preparation Method
- Dissolve 250 mg sodium carbonate in 50 ml milliQ water.
- Dissolve 300mg calcium chloride in 50 ml milliQ water under continuous sonication at 40°C.
- Add calcium chloride to sodium carbonate under sonication for 60 minutes.
- Wash the precipitate three times in a 4:2 milliQ water:ethanol mixture.
- Centrifuge at 1000 rpm for 15 minutes to precipitate calcium carbonate.
- Store freeze-dried and stored at -80°C.
“SiRNA Delivery Method”
- Transfecting cells with CaLNS-siRNA complex.
- Incubation of cells for 30-12 hours.
Questions about this Technology?
Contact for Licensing
Research Lab
Prof. Mukesh Doble
Department. of Biotechnology
Intellectual Property
IITM IDF Ref. 1970
Patent No: IN 549708
Technology Readiness Level
TRL-4
Experimentally validated in Lab;