In-vitro Method for Intracellular Delivery of Biomolecule in Three-dimensional (3D) Cell Culture Spheroids
Categories for this Invention
Category: Biotechnology & Genetic Engineering
Industry: Biotechnology and Pharmaceutical
Application: Intracellular Delivery System, Dye, Plasmid, Enzymes, Cellular Therapy, Diagnostics, Drug Development, Disease Modeling
Market: The global 3D Cell Culture Market was valued at $1.9 Billion in 2024 and is expected to reach $4.6 Billion by 2033, at 11.3% CAGR during forecast period.
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Problem Statement
- Existing methods for delivering biomolecules in 3D cell culture spheroids, suffer from low efficiency and limited penetration depth.
- Traditional delivery techniques face difficulties in achieving high transfection efficiency while maintaining cell viability, particularly in complex 3D cell culture models.
- The current methods often result in inadequate delivery, which hampers the accuracy and relevance of experimental outcomes.
- Optimizing laser power, exposure time, and spot size is crucial for intracellular delivery. Deviation leads to suboptimal results & compromise experimental integrity.
- Hence, there is a need for a method to overcome limitations of intracellular delivery techniques of biomolecules in 3D cell culture spheroids.
Technology
The technology disclosure outlines an innovative method for the intracellular delivery of biomolecules within three-dimensional (3D) cell culture spheroids. Cells are cultured in a 96-well microplate to form 3D cell culture spheroids, providing a more physiologically relevant model compared to traditional 2D culture.
Key Features / Value Proposition
- Achieves high efficiency intracellular delivery (80%-91%) within 3D cell culture spheroids.
- Compatible with various cell types, including primary cells and cancer cell lines.
- Ensures minimal cell damage and Maintains high viability with optimized laser parameters.
- Represents advancement in the intracellular delivery technique.
- Utilizes innovative gold nanostar-mediated delivery for enhanced specificity.
- Provides physiologically relevant results by mimicking in vivo tumor microenvironments.
- Offers biocompatibility and safety with gold nanostars and infrared diode lasers.
- Cost-effective and user-friendly, utilizing common laboratory equipment and reagents.
Questions about this Technology?
Contact For Licensing
sm-marketing@imail.iitm.ac.in
ipoffice2@iitm.ac.in
Research Lab
Prof. Tuhin Subhra Santra
Department of Engineering Design
Intellectual Property
IITM IDF No: 2419
IN Patent Grant No: 486848
Technology Readiness Level
TRL – 4
Experimentally validated in lab.