Method for Colocalization of Plasmonic Nanoparticles and Molecules with Plasmonic and Raman Scattering Microspectroscopy
Technology Category/Market
Manufacturing/Chemical Engineering
Industry: Nano Technology
Applications: Medicine, biological applications and optical micro spectroscopic technique.
Market: The global plasmonic materials market was around US$ 10.7B in 2022, expected rising at CAGR of 15.5% and reaching US$ 39.4B by 2031.
Image Gallery
Problem Statement
- The temporal behavior of theragnostic is vital to improve the efficacy in biological applications.
- Variety of nanoprobes are used as theragnostic of which plasmonic nanobiolabels have optical, photothermal and surface enhanced RS properties.
- However, due to complexity of biological environment resulting in multiple interfering molecules mislead the signals.
The aforesaid problems are eased with a combined  optical microspectroscopic technique as claimed in  present patent subject matter.
Technology
A method for the patent comprising:
1. A high resolution dark field condenser from Dark field scattering Micro- spectroscopy (DFSMS)
2. A Raman scattering Micro spectroscopy (RSMS)
- Wherein the high-resolution dark field condenser is integrated with conventional point-scan confocal RSMS set-up that utilizes light source with wavelength of 400-1000 nm to excite plasmonic modes of noble metal nanoparticles and a 532 nm laser to excite Raman spectra for colocalization of both plasmonic nanobiolabels and biomolecules in biological samples.
- The plasmonic nanobiolabels (silver and gold nanoparticles) is identified in chemical and biological nature using their inherent spectroscopic features. It is located in vicinity of Escherichia coli using SERS.
- The biomolecules include proteins, DNA and RNA molecules; the biological samples include bacteria (Escherichia coli), mammalian cells (HEK293 cells).Â
- The plasmonic nanobiolabels are located in HEK293 cells and biomolecules are colocalized with them.
- The state of plasmonic nanobiolabels is identified in terms of size, shape or state as a single, dimer or aggregated particles.
- The plasmonic & Raman scattering spectroscopy is performed on chemical-biological systems.
Key Features/Value Proposition
- The molecules adsorbed on Plasmonic NanoBio labels surface is used for colocalization.
- Plasmonic NanoBio labels are important due to optical, photothermal and surface enhanced Raman scattering properties.
- To eliminate the possibility of defocused imaging in PRSMS set-up, care is taken to focus the desired plane of imaging by using a laser spot used for confocal RSMS.
- Polydisperse silver nanoparticles with SPR centered around 410 nm are used as NanoBio labels in Colocalization experiments, wherein AuNPs (size ~40 nm) are the NanoBio labels in the experiments performed on chemical and biological systems using plasmonic & Raman scattering spectroscopy.
Questions about this Technology?
Contact for Licensing
Research Lab
Prof. Pradeep T
Department of Chemistry
Intellectual Property
IITM IDF Number: 1289
IP Patent Number: 419154 (Granted)
Technology Readiness Level
TRL – 3
Proof of Concept