The instant invention discloses a method for preparing a nano-scale anti-cancer compound formulation using seed oil from Cuminum spp (a type of cumin) and a non-ionic surfactant.

• Cuminum spp: Specifically mentions Cuminum cyminum as a species of Cuminum.
• The Non-Ionic Surfactant used can be tween 20, tween 80, or a combination of both.
• The seed oil composition of Cuminum spp used in the disclosed method is specified to be in the range of 4-6% v/v.
• Different ratios of seed oil of Cuminum spp and non-ionic surfactant are mixed to prepare the organic phase. The formulation chosen is one that is transparent instantly after preparation.

Method Overview:

• The method involves preparing an organic phase using seed oil from Cuminum spp and a non-ionic surfactant, then adding this organic phase drop-wise to water while stirring to obtain a nano-scaled emulsion spontaneously, which exhibits anti-cancer activity.

Optimal Formulation:

• The optimal formulation ratios are mentioned to be 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 2:8, and 3:7 (surfactant:oil). Additionally, this formulation is stable at Tepm range of 2-50°C.

Biological Activity:

• Formulation induced apoptosis (programmed cell death) in cancer cell lines and bacterial cells, indicating its potential anti-cancer and antibacterial properties.