• Lipids play crucial roles in various biological functions, and even a slight deviation in their concentration (as low as 10%) can lead to significant physiological changes and disorders, including dry eyes.
• Dry Eye Disease (DED) severity is strongly correlated with reduced lipid levels, which form the outermost tear film layer, preventing excessive evaporation.
• Current methods for lipid concentration determination are often time-consuming and expensive.
• So, there is a need to have a low cost, rapid, and efficient method and system for determining the concentration of lipid from a sample.

The present invention relates to the field of lipidomics, particularly a method and system for determining lipid concentration in a tear sample. (Fig. 2)

Sample Collection:

• Tear samples are collected from a subject. (Fig. 1)

Lipid Extraction:

• Lipids are extracted from the tear sample.

Lipid-Nanoparticle Suspension:

• A suspension of lipid nanoparticles is prepared.

 Deposition:

• The lipid nanoparticles suspension is deposited onto a pre-cleaned surface to obtain a dried deposit.

 Ring Identification:

• The method after obtaining the dried deposit comprises determining and identifying inner ring and further the outer ring. After identifying outer ring and inner ring, the method determines area fraction of the dried deposit and percentage offset of the inner ring along the diameter of the outer ring.

 Lipid Concentration Calculation:

• Using the area fraction and percentage offset data, the lipid concentration in the tear sample is calculated. (Fig. 3)