• Faecal matter (FM) contains undigested carbohydrates, bile pigments (bilirubin, stercobilin (SB) & urobilin (UB), dead leukocytes, proteins, fats & inorganic substances) in water.
• Faecal pigments (FPs) are the open tetrapyrroles formed by bilirubin degraded products(SB & UB), & both SB & UB pigments are considered as faecal pollution indicators of pathogens in water
• Based on prior arts discussion, there are a few methods discussed for detecting FPs, however the process required elaborate & expensive techniques.
• Hence, present Patent has addressed above issues efficiently.

Present patent claimed a method for detecting Faecal Pigments (FP) in water wherein Faecal Pigments includes urobilin & stercobilin in water using solid-state green fluorescence on metal salts.

Said method comprises the steps of:

• First Step describes about taking 100 mg of metal salt in the ceramic well;
• Second step describes about drop casting of 15 μL of 100 μM FPs on the surface of metal salts of first step by step-wise addition using a micropipette; and

• Third step describes about illuminating the drop casted sample from second step using a UV torch of 365 nm.