Industrial Consultancy & Sponsored Research (IC&SR) , IIT Madras
A Method for Environmental Arsenic Detection and Public Awareness using Human Cells
Categories for this Invention
Technology: a method for Arsenic detection;
Industry & Application: Healthcare, Medical kit for detection of Arsenic in water;
Market: The global water quality monitoring market is projected to grow at a CAGR of 5.51% during the forecast period (2024-30).
Image Gallery
Problem Statement
- Despite the severe health implications of Arsenic (As), an overall lack of social awareness among the As-affected population is observed.
- The problem statement discussed in the subject invention is how to provide a cellular platform (CP) sensor, which will help to create awareness about the risks of chronic As exposure effectively. This may be implemented typically in a school or a village health center in the affected region, which could store the CP. Hence, Present invention is effective to provide the solution efficiently.
Technology
Present patent describes a method & kit for environmental arsenic detection.
Method comprises the steps of:
- a cellular platform consisting of eukaryotic cells cultured on an electrospun nanofibrous scaffold made of biocompatible polymer;
- treating the cellular platform with arsenic extract;
- staining the treated cellular platform with fluorimetric probe;
- The cellular response to arsenic is measured in terms of the fluorescence response at the excitation/emission wavelengths of a fluorometric probe sensitive to reactive oxygen species (ROS) generated by arsenic.
Key Features / Value Proposition
Technical Perspective:
- Facilitates a unique, sensitive, quantitative, sustainable, & environment-friendly method for the detection of arsenic in environmental samples using cellular platform-based arsenic sensor made of human skin-cell.
- The eukaryotic cells are selected from a human cell line, wherein said human cell line include keratinocytes or HaCaT cell line from adult human skin.
- The arsenic detection includes the trivalent and pentavalent states of arsenic as well as their mixtures.
- The biocompatible polymer is polycaprolactone (PCL).
- The fluorimetric probe is 2′,7′-dichlorofluorescin diacetate (DCFDA).
- Arsenic detection output is quantitative or qualitative.
quantitative or qualitative.
Questions about this Technology?
Contact For Licensing
sm-marketing@imail.iitm.ac.in
ipoffice2@iitm.ac.in
Research Lab
Prof. Pradeep T
Department of Chemistry
Intellectual Property
IITM IDF Ref. 2405
IN Patent No. 484281
Technology Readiness Level
TRL – 4
Technology validated in lab scale.