• The diagnosis and assessment of skin disorders and the evaluation of cosmetic and anti-aging treatments currently rely on invasive procedures like skin biopsies or limited, expensive instruments with bulky instrumentation.
• Hence there is need for creating  devices that enables non-invasive, in-vivo detection of disease biomarkers, such as collagen and hemoglobin, in the subsurface layers of the skin.

The invention discloses an apparatus for measuring/ determining the collagen and/or hemoglobin on a sample (Fig. 1); It includes:

• Light Sources–for exciting and illuminating sample
• Optical Probe with a fiber optic probe and an integrated sphere
• Integrated Sphere-collect reflected light (reflectance) from the sample.
• Sensor -ensures that the probe is at the correct distance from the sample
• Spectrometer–sense the collected fluorescence and reflected light
• Data Processing Unit –processing data from the fluorescence and reflectance spectra.

1. Display Unit-displays in the form of fluorescence and reflectance spectra
2. A monochromatic light source is used to excite the sample
3. The Optical Probe consists of a fiber optic probe and an integrated sphere.
4. The fiber probe is connected to the monochromatic light source and has two main sections- Center Section having fiber cable that connects to the excitation source for the sample and Outer Section that contains a bundle of fiber cables arranged in a circular pattern at the periphery of the center section.

Further, discloses a method of operation for fluorescence and reflectance measurement that includes: