Industrial Consultancy & Sponsored Research (IC&SR) , IIT Madras
Process For Production Of Constant Molecular Weight Hyaluronic Acid By Recombinant Microbial Fermentations
Categories for this Invention
Hyaluronic acid,
Medicines,
Cosmetics
Targeted Industries
Watermarking IP design,
Electronic circuits
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Applications
Hyaluronic acid (HA) is a natural bio-polymer with wide applications in fields of medicine and cosmetics
Problem Addressed
Hyaluronic acid (HA) is a natural bio-polymer with wide applications in fields of medicine and cosmetics. Molecular weight (MW) of HA is an important quality parameter that dictates its end applications There is a need for production of HA of constant molecular weight by the manipulation of the process of fermentation.
Abstract
The present invention is related to a process for the production of hyaluronic acid with constant molecular weight (MW-HA) throughout the fermentation time with a recombinant cocci expressing has A, has B genes with or without one of has E and pgm A genes; wherein a glucostat operates and the glucose concentration is maintained at the initial concentration throughout the fermentation using a pH feedback strategy maintaining the pH of the batch fermentation at 7.0; and wherein the cocci is a recombinant Lactococcus lactis.
Advantages
1. Production of constant-MW HA over a 0.4–1.4 MDa range throughout the fermentation time using the simultaneous control of process parameter and genetic parameter.
2. Construction of recombinant strains of Lactococcus lactis to produce constant-MW HA over a 0.4–1.4 MDa range.
3. Production of constant-MW HA over a 0.4–1.4 MDa range throughout the fermentation time with the simultaneous control of process
parameter and genetic parameter and addition of modified M17 medium