An Optimized Bioprocess for Enhancing Camptothecin Yield from Endophytes
Categories for this Invention
Category – Biotechnology and Bioprocessing
Applications – Pharmaceutical, Biopharmaceutical R&D,
Industry – Pharmaceuticals and Biotechnology
Market – Global bioprocess technology market size is expected to reach $45.04 Bn by 2028 at a rate of 15.1% CAGR.
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Problem Statement
- Existing methods for Camptothecin (CPT) production from plant sources are inefficient, yielding low quantities, and are economically unfeasible.
- Camptothecin production by endophytic fungi, like Fusarium solani, decreases over time, potentially due to the absence of host stimuli in culture media.
- There is a demand for an optimized in vitro bioprocess to enhance Camptothecin production from endophytes, addressing the challenges of low yield and production attenuation.
Technology
- Enhanced Camptothecin Yield: The optimized bioprocess increases Camptothecin (CPT) production by over 60% from Fusarium solani using exogenous agents like ethanol and extracts of Catharanthus roseus.
- Efficient Extraction Process: Camptothecin is efficiently extracted from the dry biomass through a sonication and solvent extraction method, followed by quantitative analysis using RP-HPLC.
- Cost-Effective and Scalable: The process uses readily available elicitors and growth medium components, making it a cost-effective and robust method for large-scale CPT production.
Key Features / Value Proposition
1.Increased Yield Efficiency:
- Over 60% enhanced Camptothecin (CPT) production through optimized bioprocesses using Fusarium solani.
2. Cost-Effective Production:
- Utilizes readily available exogenous agents and growth mediums, reducing overall production costs.
3. Scalability Potential:
- The robust and scalable process is suitable for large-scale industrial applications, meeting growing demand for CPT.
4. Enhanced Extraction Method:
- Efficient sonication and solvent extraction protocol ensures high purity and yield of Camptothecin.
5. Versatile Growth Medium:
- Employs Potato Dextrose Broth (PDB) for optimal growth and metabolite production in endophytes.
6. Reliable Quantitative Analysis:
- Accurate quantification of CPT using RP-HPLC, ensuring consistent product quality and potency.
Questions about this Technology?
Contact For Licensing
sm-marketing@imail.iitm.ac.in
ipoffice2@iitm.ac.in
Research Lab
Prof. Smita Srivastava
Department of Biotechnology
Intellectual Property
- IITM IDF Ref. 1083
- IN 365151 – Patent Granted
- NBA/IPR/Appl/ 3514/20-21/2571
Technology Readiness Level
TRL – 4
Technology validated in lab scale.