The invention discloses a bioprocess for the production of biomass of Viola odorata L comprising:

• Preparation of inoculum comprising suspending in vitro developed callus, in growth medium(A) and growing at optimal culture conditions to form cell suspension culture, from which cells were isolated and used as inoculum;
• Inoculating the inoculum at a specific density in optimized growth medium(C) contained in bioreactor and culturing the inoculum for 12-50 days under optimal conditions of temperature, pH, light intensity, agitation, aeration and photoperiod in the presence of anti-foaming agent to form biomass;
• Harvesting the biomass after 12-50 days by filtering the contents of bioreactor through filter paper under vacuum.

The said bioprocess for the production of biomass of Viola odorata L, the bioprocess further comprises:

• Washing the harvested biomass with distilled water to form washed biomass;
• Lyophilizing the washed biomass to form lyophilized biomass;
• Homogenizing the lyophilized biomass by macerating in aqueous ethanol (50-70%) for 6 h with intermittent shaking to form homogenized biomass;
• Centrifuging the homogenized biomass to separate supernatant from cell debris;
• Collecting the supernatant and lyophilizing the supernatant to form biomass extract powder;
• Storing the biomass extract powder at -80 °C.