Perfusion Cum Compression (PCC) bioreactor chamber was designed and fabricated with the following parts.

Compression chamber:

• The acrylic compression chamber was slotted with 12 number of grew in two steps.
• First slot have a larger diameter and the lower with smaller diameter which hold cell seeded scaffolds/tissue explants.
• The first step has a cylindrical slot with diameter of 21mm and the second step has a diameter of 610mm Perpendicular to the lower grew the orifice of medium perfusion was placed.
• The both ends of the orifice (towards and from the cell seeded scaffold/tissue explants side) the curvature is increased to make the flow of medium uniform and outer end is enabled for threaded luer connectors with silicon O-rings.
• Silicon rubber tubing connected to the luer of the chamber pass through a peristaltic pump and then to the growth media reservoir.

Inflatable membrane O-ring:

• The unique inflatable membrane O-ring is fabricated using silicon-rubber.
• The one end is inflatable with compressed air and the other provisions to act as O-ring which gets suited to the larger surface diameter slot in the compression chamber.
• The dimensions of the slots vary with the cell seeded scaffolds/tissue explants.

Compression port:

• The compression port (20mm Dia)was designed like an inverted half head candle holder made of polypropylene or teflon or acrylic.
• The broad end of the compression port gets suited above the Inflatable membrane O-ring.
• On the other end of the compression port, provisions are made for connecting (barb) tubing that supply compressed air.
• O-ring through the centre an orifice of
  3 mm diameter was provisioned for supplying of compressed air.

Lock plate:

• Lock plate (2mm thick) is made of stainless steel to lock the bioreactor chamber assembly intact.
• Two plates, one at the bottom of the compression chamber and the other was suited above.
• Using stainless steel fasteners to lock plates were locked keep the assembly intact.