Industrial Consultancy & Sponsored Research (IC&SR) , IIT Madras
MASSIVELY PARALLEL HIGH THROUGHPUT SINGLE-CELL OPTOPORATION
Technology Category/Market
Category – Biomedical, Bio-MEMS device
Applications – Personalized medicine and regenerative medicine applications.
Industry- Biomedical, Cellular therapy.
Market-The global Bio-MEMS market size is forecast to reach $15.5 billion by 2027, growing at a CAGR of 11.68% from 2022 – 2027.
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Problem Statement
- Single cell intracellular delivery faces challenges due to cell membrane impermeability, necessitating disruptive methods.
- Existing techniques focus on bulk cargo delivery, resulting in low efficiency and reduced cell viability.
- Collecting data from individual cells is essential, requiring parallel high-throughput delivery methods.
- Cell culture randomness hinders monitoring and organization of single cells for research.
- There is a need for improved delivery approaches and methods for parallel single-cell therapy and analysis to advance the field.
Technology
- The present invention relates to massively parallel high throughput single cell patterning technology and intracellular delivery techniques.
- The method teaches use of parallel single-cell patterning technique using SU-8 membrane and nano-second pulsed laser espouse on single-cell with micro-dish pattern device structure (Fig. 1, 2 & 3).
- The platform is able to effectively deliver different (small to large) cargo in a different cell type with high transfection efficiency and high cell viability at parallel single-cell resolution. Fig. 4.
Key Features/Value Proposition
Massively Parallel Single-Cell Patterning:
- The method offers a unique approach to pattern single cells in a massively parallel high-throughput fashion, allowing for precise control and organization of individual cells.
High Transfection Efficiency:
- It achieves high transfection efficiency and cell viability, enabling the effective delivery of various cargo sizes to different cell types.
Versatile Cell Patterning:
- The platform can accommodate single-cell patterning with varying hole sizes, making it adaptable to different cell types and research needs.
Questions about this Technology?
Contact for Licensing
smipm-icsr@icsrpis.iitm.ac.in
ipoffice2@iitm.ac.in
Research Lab
Prof. Tuhin Subhra Santra
Department of Engineering Design
Intellectual Property
- IITM IDF Ref. 2005
- IN 202041031463
- PCT/IN2021/050706 – Published
- US18006098
Technology Readiness Level
TRL – 3
Proof of concept stage.