2D Substrate Protein Micropatterning Process for Cell Alignment
Categories for this Invention
Technology: Process for fabricating a SU 3D structure on a silicon substrate
Category: Precise single cell analysis
Industry: Biomedical industry
Application: Tissue engineering ,nanotechnology
Market: The global market size was estimated to be worth $3.5 billion in 2023 and is poised to reach $7.1 billion by 2028, growing at a CAGR of 15.3% from 2023 to 2028
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Problem Statement
- Cells interact with the Extracellular Matrix (ECM) through receptors like Integrin, sensing mechanical and chemical stimuli.
- Organs have unique structures and alignments, making 2D and 3D patterned microenvironments crucial for cell growth in tissue-engineered organ substitutes.
- Tissue engineering techniques create customized microenvironments for cell alignment, varying from organ to organ.
- Techniques include electromagnetic fields, electrophoretic force, nanofiber scaffolds, 3D gels, and microstructure substrates.
- There is no techniques using chemical stimulus to achieve cell affinity and align cells on a substrate.
- The need for an improved 2D substrate protein micropatterning process exists to align cells in a specific pattern by providing chemical stimulus, achieving cell affinity to the specific stimulus.
Technology
2D Substrate Protein Micropatterning Process:
- Forming mold using SU8 3D structure on silicon substrate.
- Casting polydimethylsiloxane (PDMS) stamps using SU8 3D structure.
- Cleaning silicon substrate with Piranha solution at 80°C for 10 minutes and washing in deionized water.
- Dried with nitrogen blow, kept at 120 °C for 5 minutes for dehydration in order to form the SU8 3D structure
- Casting PDMS pillar array using mold.
- Aligning cells in a specific pattern using 2D substrate.
- Achieving cell affinity to specific chemical stimulus
Key Features / Value Proposition
Structure
- A mold is formed using a SU8 3D structure on a silicon substrate for casting polydimethylsiloxane (PDMS) stamps.
- The SU8 3005 (Micro chemical) is selected for making the 3D mold.
- The SU8 3D structure is spin-coated, soft-baked, patterned, hard-baked, and developed in a SU8 developer.
Cell Alignment
- Innovative Cell Alignment Process in Extra Cellular Matrix (ECM) Structure
- Improved 2D substrate protein micropatterning process for cell alignment.
Cell affinity
- Provides chemical stimulus to cells, enabling them to align in a specific pattern and achieve cell affinity.
Toxicity
- The process is nontoxic, easy to implement, and can pattern heterogeneous cell lines.
Performance
- Efficient analysis of genetic disorder for the patient at a single cell level
- Precise genome and transcriptome analysis at the single cell level, provide functional consequence of mutation and copy number variation of cells
Questions about this Technology?
Contact For Licensing
sm-marketing@imail.iitm.ac.in
ipoffice2@iitm.ac.in
Research Lab
Prof. Tuhin Subhra Santra
Department of Engineering Design
Intellectual Property
IITM IDF Ref. 1966
Patent No: IN 486480
Technology Readiness Level
TRL- 4
Experimentally validated in Lab